Ca2930767a1 production of thapsigargins by thapsia cell. Pmc free article rasmussen u, broogger christensen s, sandberg f. The effects of thapsigargin and siphonodictyal b1 on intracellular calcium levels were not distinguishable in pharmacological experiments conducted with caffeine, ryanodine, muscarine, and thapsigargin in calcium free and calciumcontaining buffer, although thapsigargin was effective at lower concentrations. An ethnopharmacological survey of plants used in traditional diabetes treatment in southeastern algeria ouargla province home. It grows to about 70 to 190 cm 28 to 75 in in height. Show the yellow inflorescence of thapsia garganica, and the mature infructescence of the species. Their center of diversity is around the western mediterranean, extending into the atlantic coasts of portugal and morocco. The scanned text used to begin the current translation project was obtained from. Thapsia garganica, medicinal plant, essential oil, gcms. Files are available under licenses specified on their description page. The mediterranean plant thapsia garganica dicot, apiaceae, also.
Identification and characterization of a kunzeaol synthase from thapsia garganica. Usda, ars, germplasm resources information network. This page was last edited on 29 august 2018, at 09. Effect of solvent polarity on the content of biomolecules. In 1978, the first pharmacological effects of thapsigargin were established and the full structure was elucidated in 1985. Micropropagation of thapsia garganica a medicinal plant a murashige and skoog agar medium supplemented with 0. The two macrocyclic lipids 2hydroxymethyl1,4dioxacycloicosane5,20dione 1 and 3hydroxy1,5dioxacyclohenicosane6,21dione 2 have been isolated from the fruits of thapsia garganica. Thapsia garganica showed high flexibility of functional traits and shifts in the timing of phenological events in response to. Highlights thapsigargin is a very useful tool to study calciumdependent signaling pathways. For centuries preparations containing resin from the root of thapsia garganica l. Thapsigargin is a potent inhibitor of the sarcoendoplasmic reticulum calcium atpase pump, inducing apoptosis in mammalian cells. It is used in a poultice as an antitussive treatment of acute bronchitis and pneumonia, in preparations with milk or oil taken orally to treat common lung diseases, and with the direct application of root sections for the soothing of dental pains.
Materials and methods plant materials the aerial parts of ballota pseudodictamnus, salvia fruticosa and thapsia garganica were collected from the aljabal alakdar area, libya, in april 2010. Their structures were established by physical methods, including xray analysis of transtaganolides a and b. Effects of gamma irradiation and comparison of different extraction. Antioxidant and antimicrobial properties of five medicinal. The antioxidant activity extracts was determined using the two free radical. Halima nebeg, khedidja benarous, talia serseg, asma lazreg, hafidha hassani and mohamed yousfi affiliation. This mechanism has been used to develop a thapsigarginbased cancer drug first by genspera and later inspyr therapeutics westlake village. An umbelliferous plant, growing in southeastern europe, and well known to the ancients, named from the isle of thapsos, where it was obtained. Reinvestigation of essential oil content of thapsia. Identification and characterization of a kunzeaol synthase. Thapsia garganica is a very famous medicinal plant known especially for its. Isolation of thapsigargin from thapsia garganica l.
Thapsigargin tg is extracted from the plant thapsia garganica l. Tg is the most widely employed serca inhibitor michelangeli and east, 2011 and can inhibit serca activity with an ic 50 in the subnanomolar range sagara and inesi, 1991. It has pinnate hairy leaves with sheathlike petioles. Reinvestigation of essential oil content of thapsia garganica grown in the east of algeria pdf.
Acclimatisation was low52% plantlet survivalas micropropagated plantlets were highly susceptible to. We developed a two steps procedure coupling accelerated solvent extraction to cpc. Theophrastus speaks of its root as emetic and purgative. The sesquiterpene lactone thapsigargin is found in the plant thapsia garganica l. Thapsia, commonly known as the deadly carrots, is a small genus of poisonous plants in the. They develop into fruits with four wings characteristic of the genus. The plant is also strongly rubefacient, producing blisters and intense itching. Seeds, leaves and roots of thapsia garganica as a source of new potent lipases inhibitors. Thapsigargine and thapsigargicine, two new histamine liberators from thapsia garganica l. Pdf micropropagation of thapsia garganicaa medicinal plant. The leaves are dilated at base, onehalf clasping the. Thapsigargin, a tumor promoter, discharges intracellular. This is the first time that a 7methoxy4,5dihydro3hoxepin2one ring has been found in a natural product.
Tethered lipids are unprecedented in the plant kingdom. The mediterranean plant thapsia garganica dicot, apiaceae, also known as deadly carrot, produces the highly toxic compound thapsigargin. Thapsia laciniata rouy apiaceae produces irregular and regular sesquiterpenoids with thapsane and guaiene carbon skeletons, as found in other apiaceae species. Plants were identified by dr mohamad abouhadra, faculty. Thapsia villosa, commonly known as the villous deadly carrot, is a species of poisonous herbaceous plants in the genus thapsia.
This book is available for free download in a number of formats including epub, pdf, azw, mobi and more. Effects of gamma irradiation and comparison of different. Sesquiterpenoids in thapsia sesquiterpenoids are widespread within the genus thapsia, however, it is thapsigargin and the group of similar compounds, guaianolides, which are of most interest t able 1. Thapsigargin and nortrilobolide are sesquiterpene lactones found in the mediterranean plant thapsia garganica l. All structured data from the file and property namespaces is available under the creative commons cc0 license. Five libyan medicinal plants thapsia garganica, hammada scoparia, euphorbia serrata, hyoscya mus albus and retama rateam were selected to evaluate their biological activities. Seeds, leaves and roots of thapsia garganica as a source. Four novel and unusual c19 compounds from thapsia transtagana, named transtaganolides a. Localization and invivo characterization of thapsia garganica. As a copy of the translation by julius berendes in 1902, it has entered the public domain in the united states and various other countries. The present invention relates to a method of producing sesquiterpene lactones of the thapsigargin family, the method comprising the steps of. Sesquiterpenoids from thapsia species and medicinal. Thapsia garganica allelopathic potentialities explored for. Molecules free fulltext thapsigarginfrom thapsia l.
Thapsia garganica phytotoxicity was studied on lactuca sativa l. Use of a temporary immersion bioreactor system for the. Thapsia garganica thapsia garganica thapsigargin induces proliferation independent apoptosis in prostate cancer cells n hn h o oh c o o h o oh o c o 3c o o c o o h o o o oh o 2n o h o c h psma is an nacetylated alpha psma is a folate hydrolase o naag naa glutamate 24aa extracellular707 aa nh 2 intracellular 19 aa psma. Thapsia garganica in the germplasm resources information network grin, u. The flowers are yellow in color and borne on compound umbels. The plant has been considered specific in treating pain, though caution is advised since it is poisonous to some mammals. Thapsigargin amount in different specimens of thapsia garganica l. A very potent and highly selective inhibitor is thapsigargin tg, a sesquiterpene lactone derived from the plant thapsia garganica rasmussen et al. Thapsia garganica wikispecies, free species directory. A transcriptomic analysis utilizing illumina nextgeneration sequencing enabled the identification of novel genes involved in the biosynthesis of terpenoids in thapsia. Standard antiproliferative chemotherapy is relatively ineffective against slowly proliferating androgenindependent prostate cancer cells within metastatic sites. Pdf essential oil composition of the root bark of thapsia. Thapsia, commonly known as the deadly carrots, is a small genus of poisonous plants in the family apiaceae.
The antioxidant activity was estimated using 2, 2di phenyl1picrylhydrazyl dpph as free radical scavenger. Allelopathic effect of the isolated allelochemicals on lactuca sativa l. A taxonomic genus within the family apiaceae deadly carrots. Their total phe nolic and flavanoid contents were assessed. Transcriptome analysis of thapsia laciniata rouy provides. Thapsia silphion is thought to be identical with thapsia garganica, is found on the mountains near the site of ancient cyrene, and is said to have yielded the gum resin to the ancients as laser cyrenaicum or asa dulces, the greek name being silphion.
Ali h, christensen sb, foreman jc, pearce fl, piotrowski w, thastrup o. An expeditious, multigram isolation protocol for the. A manual of organic materia medica and pharmacognosy. Thapsia garganica is a very famous medicinal plant known especially for its therapeutic effects such as diuretic, emetic and purgative. Medium free of pgrs promoted rooting at a low incidence.
Localization and invivo characterization of thapsia. Effect of solvent polarity on the content of biomolecules and antioxidant activity of thapsia garganica apiaceae for the first time, we have evaluated the antioxidant properties of principal secondary metabolites of methanol, ethyl acetate, hexane and water extracts of thapsia garganica leaves. Prostatespecific antigenactivated thapsigargin prodrug. Isolation and identification of four bioactive allelochemicals. Thapsia garganica firrastrina comune flora italiana. An ethnopharmacological survey of plants used in traditional diabetes treatment in southeastern algeria ouargla province. You can also read the full text online using our ereader. Reinvestigation of essential oil content of thapsia garganica grown. Stock cultures of thapsia garganica grown on murashige and skoog agar medium 1962 ms 0. A resin is extracted with alcohol from the bark of the root.
1237 775 1512 1007 1159 1235 152 378 455 209 255 1592 1212 883 1124 863 1108 358 531 280 985 450 145 716 594 421 1483 429 460 875 44 778 1143 369 512 1469 818 1223